Re: [DIYbio] Re: PCR trouble

I ran several reactions with 7.5 minutes of elongation per cycle. Also tried several dilutions of my miniprep, I ran a 1x 100x 500x and 1000x dilution. I also ran the plasmid in the gel with a XhoI digest. The digest didn't show up on the gel at all, the uncut plasmid made the right band, and I got the same small product on the dilutions as before except for the 500x which had no band.

So it looks like the template was present, the dilutions didn't affect it or the extra extension time. I'll be running it again once the Q5 gets here on Monday. It does come with buffer I asked. I'm not sure if that's all I'll need though.

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