Your dilution looks fine.
Less than 100bp is usually primer dimers. Check your annealing temp, make sure you have enough template. As Tom said you do have a pretty big amplicon, so it might take some fine tuning.
Could you post a picture of your gels?
You know your miniprep worked?
On Tue, Mar 1, 2016 at 9:55 PM, David Ishee <midgardkennels@gmail.com> wrote:
I don't know, it's less that 100 bp dimers are possible. My target and product would be much bigger.
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