On Thu, Apr 28, 2016 at 12:00 AM, Patrik D'haeseleer <patrikd@gmail.com> wrote:
I have a pretty good idea how to build one but I wanted to check if this had already been done and if not if it is actually useful to anyone.
I do remember Nathan and some other folks discussing using piezo lighters to do electroporation. That would be the $2.97 version :-) Not sure how repeatable and standardized you could make that though.
Yeah I've looked at it and it might be worth trying. The voltage is in the right range but the pulse duration is much shorter than what I've seen mentioned in any electroporation protocol.
Given how dirt cheap flyback transformers and other high voltage gear are on my favorite online discount site, perhaps that might be a better way to go.
I'd probably use something like this:
to charge a bunch of 330v capacitors coupled both in parallel and serial (charge pump) to give something that can be charged in parallel to between 300v and 833v and discharged at between 300v and 2500v over a period of 0.1 ms to 5 ms using 2k5v solid state relays.
It'd be fairly easy to add conductance measurement to alert the user if spiking is likely to occur (e.g. too high salt content), which would have the bonus of making the electroporator double as a conductance meter.
It'd also be simple enough to add measurement of the discharge curve using a voltage divider and the built in ADC of an atmega chip.
Add an ESP8266 and you'd be able to connect via wifi+web to download and view the conductance measurement and discharge graph for use in your scientific article.
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marc/juul
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