Re: [DIYbio] Re: DIY electroporator

There was an unfortunate split of those conversations into another
mailing list (http://lists.cibolo.us/pipermail/open_electroporator/)
which subsequently crashed or something, and all content was lost
publicly. There was definitely more discussion on safety, pulse
shaping, pulse lengthening. I have these discussions still here in my
gmail account... but would need a few hours to sit down and back them
up, then convert and re-upload them somewhere (probably the diyhpl.us
git-powered wiki).

Is electroporation preferable? From the first time I used it, my first
summer internship, I was hooked. I thought "screw chemical
transformation" for the, in my opinion, more elegant, tuneable,
eco-friendly/greener-chemistry, faster, wider species coverage (you
just vary the pulse shape/voltage, maybe the salt content of the
buffer)... and higher efficiency to boot.

Why isn't it more prominent? Because no one has made a cheaper or
open-source version yet. If I'd have figured this out when I was still
in college, I probably would have built (at least) a unit for each lab
at school.

On Thu, Apr 28, 2016 at 1:53 AM, Marc Juul <juul@labitat.dk> wrote:
>
>
> On Thu, Apr 28, 2016 at 12:00 AM, Patrik D'haeseleer <patrikd@gmail.com>
> wrote:
>>
>> I do remember Nathan and some other folks discussing using piezo lighters
>> to do electroporation. That would be the $2.97 version :-) Not sure how
>> repeatable and standardized you could make that though.
>>
> Yeah I've looked at it and it might be worth trying. The voltage is in the
> right range but the pulse duration is much shorter than what I've seen
> mentioned in any electroporation protocol.
>
>>
>> Given how dirt cheap flyback transformers and other high voltage gear are
>> on my favorite online discount site, perhaps that might be a better way to
>> go.
>
>
> I have a pretty good idea how to build one but I wanted to check if this had
> already been done and if not if it is actually useful to anyone.
>
> I'd probably use something like this:
>
> http://www.ebay.com/itm/High-Voltage-DC-DC-Boost-Converter-5V-12V-Step-up-to-300V-1200V-Power-Module-/201472584304
>
> to charge a bunch of 330v capacitors coupled both in parallel and serial
> (charge pump) to give something that can be charged in parallel to between
> 300v and 833v and discharged at between 300v and 2500v over a period of 0.1
> ms to 5 ms using 2k5v solid state relays.
>
> It'd be fairly easy to add conductance measurement to alert the user if
> spiking is likely to occur (e.g. too high salt content), which would have
> the bonus of making the electroporator double as a conductance meter.
>
> It'd also be simple enough to add measurement of the discharge curve using a
> voltage divider and the built in ADC of an atmega chip.
>
> Add an ESP8266 and you'd be able to connect via wifi+web to download and
> view the conductance measurement and discharge graph for use in your
> scientific article.
>
> --
> marc/juul
>
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--
-Nathan

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