Re: [DIYbio] New To DIYBio

* correction: luciferase will glow constantly as long as it has an ATP source. If fed intermittently it will flash

On Sun, Sep 10, 2017 at 10:13 AM Skyler Gordon <skg...@gmail.com> wrote:

To be completely honest in this particular setting neither of these proteins will show any presence in the plant unless you're a whiz with protein signaling.

If you can get luciferin to be expressed in the right tissue, correctly fold, and then maybe be stored somewhere (some vacuole or empty lysozyme) you'll still run into the issue of having to feed it ATP to cause the glowing. And even then, it will glow constantly.

(Check out pyrosequencing if you want to get a detailed description of how luciferase works)

There is the possibility that you can control the expression via a gene signaling system like the Lac operon, but I'm not overly familiar with plant systems.

You could add the gene several times to make sure it's in the correct section of the genome (Agrobacterium are somewhat undirected in their insertion methods) and try to include a genetic signal that causes expression / is turned on only in the flowering tissue.

GFP is most commonly used as a protein conjugate in order to observe where the protein is located in the cell. So the choice is really going to depend on what you're using it for.

When you are trying to just make something glow for the sake of curiosity / wonder I would suggest trying to use as little ATP as possible. For the health of the plant.

I hope this helps.

-SG

On Sat, Sep 9, 2017 at 8:11 PM a a <haitath...@gmail.com> wrote:

Correct me if I'm wrong, but wouldn't luciferase be preferable if it didn't rely on flourescence to glow (doesn't need uv light)?

On Tuesday, August 15, 2017 at 5:49:57 PM UTC-7, Skyler Gordon wrote:

Luciferase is going to need other proteins to function and is also going to consume ATP to cause the glow it creates. GFP is a better route because it has fluorescence and doesn't just glow/produce light like luciferase would

-SG

On Sun, Aug 13, 2017 at 12:12 PM Matt Tucker <mathew....@gmail.com> wrote:

How about luciferase?

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