Just to make a correction to myself from before, .1 ul E.coli would contain 100 ug of the bacterium, not 100 ng. You would want to dilute it 1:1000 before re-dilution for injection - yikes! Don't try at home folks!
On Sunday, April 5, 2020 at 4:02:06 PM UTC-7, Zonie deep wrote:
-- On Sunday, April 5, 2020 at 4:02:06 PM UTC-7, Zonie deep wrote:
Hola it's very easy to highly purify expressed proteins using affinity tags and resins that bind the tag. And very easy to make relatively large quanities.
Express the a portion of the receptor binding domain of the spike protein. There's data on best epitopes.
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