https://www.sciencedirect.com/science/article/pii/S0166354216301826
MERS-CoV genome is assembled similar to other CoVs, and the gene coding for S is located in the one-third of the genome at the 3′ end (Fig. 2A). S is a type I, trimeric, transmembrane protein located at the surface of the viral envelope, giving rise to spike-shaped protrusions from the virion. S is 1353 amino acids in length, heavily glycosylated (with 21 predicted N-linked glycosylation sites), and consists of a large ectodomain and a short cytosolic tail (Fig. 2B). The S proteins of CoVs can be divided into two functional subunits (Xia et al., 2014): the N-terminal S1 subunit forms the globular head, and the membrane-embedded C-terminal S2 region forms the stalk region. S1 is involved in receptor recognition and binding and is less conserved compared to the stalk. Two discrete, independently folded domains are located at the N- and C-termini of S1 and are able to bind receptors.
On Apr 5, 2020, at 4:02 PM, Zonie deep <deckeraaa@gmail.com> wrote:
You know if we are desperate enough it would be quite easy to make your own purified recombinant covid spike protein in bacteria. This could be used for a vaccine without too much regulatory. Protein could be made in a few weeks. Need to be sterile of course, dosage worked out. I've only immunized rabbits with these types of preps. Always get good antibodies,no harm to rabbits.
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