Re: Didymium standards

That spreadsheet was made available under GPL, so I have attached it.

-Rob

On Sun, Nov 27, 2011 at 3:27 PM, Simon Quellen Field <sfield@scitoys.com> wrote:
> The slit is not the limiting factor in resolution.
> The slit I use is 250 microns, and I get sub-nanometer resolution from
> my optics. And sensitivity is a function of the light source behind the
> slit, which we can simply increase, in the case of absorption sampling.
> The holmium perchlorate data is here:
> "http://www.starnacells.com/d_ref/d_wl/HL.html"
> Their chart starts at 230 nm, which just happens to be the highest frequency
> interesting for the DNA quantification project.
> This article:
> "http://www.sciencedirect.com/science/article/pii/S0301462207002943"
> claims to have an Excel spreadsheet that predicts the UV spectra of any
> oligodeoxynucleotide sequence between 215 nm and 310 nm, but I haven't
> sprung for the $31.50 since I'm just kibitzing on Nathan's project. But he
> might
> want to read it.
> Holmium glass (not as easy to find as dydimium glass) might be an excellent
> calibration source for 260/280 ratios. Whether it is better than dydimium
> glass
> for that is questionable, however, since all you really need is a
> reproducible
> ratio.
> You might also play with neodymium glass, which is easy to find at your
> local
> hardware store or at Amazon.com. A four dollar calibration source is perfect
> for a DIY spectrometer.
>

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