Re: how can i check a gene is either expressed or not or over expressed after applying the drugs

If you're trying to observe the effects on a native protein in its
natural context, that rules out a lot of tricks. It leaves you with
variants of PCR; the most obvious being reverse-transcription PCR.

Reverse transcription PCR will help you to determine whether the mRNA is
being transcribed. That doesn't necessarily mean the protein is being
translated, but usually it does.

To do that, you're probably best getting and using a kit if you're not
familiar with RNA handling; the kits use column-based procedures and
come with RNAse-killing reagents and DNAses to kill background DNA.
There's all sorts of voodoo depending on who you talk to, but I'd at
least recommend wearing a facemask to prevent microdroplets of nasal or
oral moisture from entering your samples; full of RNAse!

On 01/12/11 07:24, debadutta bhoi wrote:
> i m actually doing project on a drug effect on cancer cells . the drug
> is actually a Histone Deacetylase Inhibitor. so when i can i monitor
> the drug is actually inhibiting the protein and how can check the
> some particular gene is expressed.

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