[DIYbio] Re: DIY tube holder for vortex mixers

Why couldn't you use the foam block on a vortexer idea that I posted
about? I see that in the picture the tubes are horizontal, you could
just cut slots in the foam so the tubes are horizontal rather than
vertical. This would be easier than mounting an inverse dremelfuge to
a vortexer.


On Feb 1, 3:49 am, David McWilliam <dmcwill...@gmail.com> wrote:
> I want to extract DNA from fresh/dried plant material. One technique is to
> macerate the tissue by placing it in a tube with beads and mounting it on a
> vortexer. There are commercial kits (http://www.geneworks.com.au/Product/ProductDetail.aspx?p=32&pid=9582) which
> require adaptors for some vortexers (http://www.mobio.com/vortex-and-vortex-adapters/vortex-adapters-for-v...).
> I believe a dremelfuge tube holder mounted on a vortexer (mine is 1.5"/38mm
> dia) would work as well. My thoughts were to get a piece of 1.5" plastic
> tube to fit over the vortexer hub and cut slots in the top to correspond
> with the radial arms of the dremelfuge. The tubes may need restraint to
> avoid being shaken out (maybe a plastic wedge between the cap and the hub).
> I haven't tried any of these ideas yet - still looking for a suitable piece
> of plastic tube.
> DMcW
>
> On 1 February 2012 16:08, CodonAUG <elsbe...@gmail.com> wrote:
>
>
>
>
>
>
>
> > I am not familiar with your technique, do you have any more details
> > about it?  Maybe I can offer some ideas.
>
> > On Jan 20, 2:26 am, David McWilliam <dmcwill...@gmail.com> wrote:
> > > Great post. I've been looking for something like this for macerating
> > plant
> > > material in bead beater tubes prior to DNA extraction. I've just  bought
> > a
> > > dremelfuge which arrived today and am thinking of ways to attach it to a
> > > vortexer. The horizontal tube position would help maximise the bead
> > > vortexing although it may need something to stop the tube caps from
> > > popping. Maybe a vortexer modification of the dremelfuge - Cathal?
> > > DMcW

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