Hi fellas, original author here.
Nathan, the overlapping area of the electrical field and the plasmid
injection site is what determines the location of expression; In this
case, the major muscle groups. Some immune cells passing through the
injection site at the time may also get transfected, but the vast
majority will be local muscle fibers.
Generally speaking, making a mouse inhale a plasmid will generate some
gene expression, perhaps even in the liver, but mice are much more
receptive to naked DNA transfection than larger mammals. While the
amount of transgene required for a visible effect here isn't super
high, the efficiency increase of at least 100x that I expect from
electroporation will likely be necessary, as I prefer as few
injections/electrocutions as possible.
Mad_casual: it's true that this could cause just as much wear & tear
on the heart and tendons as muscle gained from weightlifting, though
possibly less since the growth occurs without the strenuous exercise.
I am in fact 20(-something), 6'2", and consider myself bulletproof, so
I'm willing to accept the risks there :P
On Mar 1, 10:43 am, mad_casual <ademloo...@gmail.com> wrote:
> On Mar 1, 11:30 am, Nathan McCorkle <nmz...@gmail.com> wrote:
>
> > How would the plasmid target muscle cells only, not including the heart?
>
> Myostatin and MyoD are skeletal muscle genes and electroporation is
> local. IMO, the concern is the other way; non-genetic DIYBio muscle
> growth has been going on for 60 yrs., there's plenty of non-clinical
> evidence to suggest that rapid growth of muscle and decades of walking
> around with tons of extra muscle (pharmacologically induced or not)
> doesn't do your heart or connective tissues any favors. If I were 20
> yrs. old, 6'2" and bulletproof, I'd probably try this, once my kids
> are out of the house and I'm having trouble keeping up in the gym, I
> might try something like this. Right now, it just seems like a bad
> idea.
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