They definitely don't divide; nature sperm cells are terminally differentiated and hyperspecialised.
As to survival, common wisdom suggests around 7 days in the relatively hostile uterine environment, so I'm sure clever culture methods could keep them at least that long.
However, living sperm are not necessarily viable for their intended purpose..without a lot of love, they'll begin to fray and mutate. If you're considering IVF, you must consider the responsibility you'd hold over any mutant babies!
Nathan McCorkle <nmz787@gmail.com> wrote:
>I'm looking for the length of time until death of spermatozoa isolated
>from ejaculate, incubated in mammalian cell culture media. Even a
>paper showing time post-ejaculation vs in-vitro fertilization (IVF)
>success would be good. I basically want to know how long these suckers
>can stay alive outside the body, though I'm pretty sure they don't
>divide.
>
>This could lead to things like glowing ejaculate in a tube... or
>potentially @home IVF. A lot more equipment would need developed for
>the latter to be @home... but heck, let's not be thought police on
>far-out ideas.
>
>
>This gives some simple vitality assays, and isolation methods, but
>doesn't really show a time vs vitality curve
>http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0006446
>
>This is just kind of weird... I wonder what would happen if they
>implanted:
>http://onlinelibrary.wiley.com/doi/10.1002/mrd.1120160108/abstract
>
>--
>Nathan McCorkle
>Rochester Institute of Technology
>College of Science, Biotechnology/Bioinformatics
>
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