I've successfully used Epoch spin columns for multiple applications, including PCR clean up, removal of restriction enzymes, gel extraction, and minipreps, A friend of mine gave me the used columns from his lab (which they don't recycle) and I make all the solutions myself. They are easy to recycle as well. When your finished with them, let them soak for 24-48 hrs in 1M HCl, wash 5X with dH20, and 2X with TE, and they should be good to go. You can also make your own columns if you happen to have any Whatman GF/F paper. Just remove the glass filter that is in the column, cut out a very small piece to replace it with (I usually scrunch up an ~1.0 X 1.0 cm piece and stuff it in the column with a pipette tip.
On Mon, Mar 26, 2012 at 6:16 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
On Mon, Mar 26, 2012 at 8:51 PM, Dakota Hamill <dkotes@gmail.com> wrote:Epoch sent me samples for free... thought they probably can't sustain
>
>> Epoch is a good source.
>>
>> http://www.epochlifescience.com/Product/SpinColumn/minispin.aspxThey ship
>> to your house. They arent as re-usable as the Qiagen columns, a higher
>> failure rate, but the price is good.
>
>
> Thanks for that link Tom, those prices are awesome! That's 50% cheaper than
> anywhere else I've found them for the 50 kits. Sequencing looks cheap too,
> $5. I was just about to spend $80 on a Qiagen kit, Epoch is only $30.
>
doing that much.
--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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