On Thu, Mar 1, 2012 at 9:21 PM, Max Berry <maxberry@gmail.com> wrote:
> Hi fellas, original author here.
>
> Nathan, the overlapping area of the electrical field and the plasmid
> injection site is what determines the location of expression; In this
> case, the major muscle groups. Some immune cells passing through the
> injection site at the time may also get transfected, but the vast
> majority will be local muscle fibers.
>
> Generally speaking, making a mouse inhale a plasmid will generate some
> gene expression, perhaps even in the liver, but mice are much more
> receptive to naked DNA transfection than larger mammals. While the
> amount of transgene required for a visible effect here isn't super
> high, the efficiency increase of at least 100x that I expect from
> electroporation will likely be necessary, as I prefer as few
> injections/electrocutions as possible.
Yeah but how often would you have to inject/electroporate? I think
most people would rather inhale a few milligrams of DNA aerosol
through their nose every once in a while. It also seems like with
electroporation of muscle you'd get uneven expression, and thus
develop muscle deformities/disfigurement.
--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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