Re: [DIYbio] What are common basepair mismatches?

G-T is relatively common, I gather.

Nathan McCorkle <nmz787@gmail.com> wrote:

>I'm working on a protocol involving DNA hybridization and ligation of
>short sticky-ends (about 3 bp), and I'm concerned with the
>possibility of sticky-end mismatches. Using nucleotide kinase and
>phosphatase I can prevent mismatches from ligating, but if the risk is
>low, leaving these steps out decreases the reagent bill and time cost
>significantly.... but I need to evaluate the risk so I can choose if
>the level is acceptable or not.
>
>So of the possible mismatches, which are most prevalent/likely (even
>if statistically the probability is low)?
>
>Basepair mismatch possibilities:
>GA, GT, GG, CA, CT, CC,TT, AA
>
>
>--
>Nathan McCorkle
>Rochester Institute of Technology
>College of Science, Biotechnology/Bioinformatics
>
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