[DIYbio] Re: Bioluminescent yogurt (Again!)

@Nathan: By upp-counterselectable gene replacement system, I meant the method they used for gusA3 for the insertion. I looked into the references and understand that the method requires a plasmid.


@Cathal: Acidophilus can be used a a primary yogurt culture with thermophilus, much like bulgaricus. But it has a longer generation time, I believe (my results say so). Also, this is an article in which they used acidophilus with thermophilus as the primary culture http://www.ajcn.org/content/71/3/674.full

@Nathan: Thanks for sharing those links. Some of them were really useful. :)

@Cory: The wavelength naturally should be in the blue region. But with modifications they can be altered.
As for the toxicity, we will have to check the tetradecanal-aldehyde and look for its toxicity. But if luciferase metabolizes actealdehyde as well, which is present in normal yogurt, we could expect it to be less toxic. Conclusively, it shouldn't be toxic.

Cheers,

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