[DIYbio] Re: DIYBIO events this Saturday!

Hey folks!

We will be doing/learning about biology this Saturday at sprout!  COME ONE COME ALL!  We will start with a general meeting/introduction to BOSSLAB, and then we will make kombucha and start the biotech 101 class.  Everyone is welcome to come and watch, but if you want to participate in the biotech 101 class, or want materials for making kombucha, please buy an event ticket.  Ticket for biotech 101 here, kombucha supply ticket here.  The links should take you to a wepay page.

1) Open meeting time+making kombucha:
Agenda:
-Introductions
-Organize sea slug and chiton projects, as well as anything else that is brought to the meeting (synbio, gfp in higher order organisms, hardware parties)


2) DIY-Biotech 101 class begins!  This will be held on Saturdays from 1-5, although some classes may be longer or shorter depending on what we are doing that day.  This is a four part class, with this schedule:

May 5 : Learn lab technique and most importantly, SAFTEY! This will be the first day of class, and we will learn how to use the tools at bosslab, what they do, and aseptic technique!

May 12: Last week we grew up bacteria...This week we will introduce foreign DNA- a gene from a jellyfish - into them. In a few days we will see them turn green!

May 19: We put the DNA in, so we should be able to take it back out! We will extract high-quality plasmid DNA from bacteria, and talk about how we would do it for different animals or kinds of DNA.

May 16: You have options here! We can either do this:

Protien extraction! Now that the bacteria are making this protien, how do we extract it from the bacteria? Chromatography of course! Learn about how the properties of protiens can be used to seperate them from each other.

Or this!

Now that we have the DNA, it would be cool to visualize it, since it is pretty much invisible in solution. In this class, we will learn how to do agarose gel electrophoresis, and how people can "see" DNA!

Please join us!

Avery