Re: [DIYbio] A question about homologous recombination

> Is there a chance that it is inserted into the chromosome (say attached to
> LuxA), and can other sequences get lost because of the substitution (e.g.
> LuxR; regulation, decreasing light output)?

Plasmid - chromosome recombination is certainly a possibility in large
homologous stretches. However, in gram-negative bacteria it isn't all
that efficient. It happens very rarely.

When people do "recombineering" in e.coli they do so by first
expressing three lambda phage recombination genes and then introducing
the thing-to-swap-in as a -linearized- version of the DNA. Linear DNA
swaps in much more efficiently.

Yeast, on the other hand, swaps exogenous DNA into its chromosome with
surprising efficiency, which is why yeast genetics is the most
advanced among any organism - it just works.

-a

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.