Harsh,
On Apr 6, 2012 9:32 AM, "medminus9" <harshsethia1989@gmail.com> wrote:
>
> @Nathan: By upp-counterselectable gene replacement system, I meant the method they used for gusA3 for the insertion. I looked into the references and understand that the method requires a plasmid.
>
While they used a plasmid, it was merely a byproduct of their prior cloning procedures, linear DNA (E.G. synthetic DNA) would have worked just the same.
> Conclusively, it shouldn't be toxic.
I don't think we can say that so easily... People complaint that the Bt protein, a single protein, gives them weird problems
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