On Thu, Apr 5, 2012 at 3:47 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
> Chromosomal Integration via Homologous recombination of L. delbrueckii:
> http://www.google.com/patents/US5747310
>
> Electrotransformation of L. delbrueckii w/plasmid:
> http://www.ncbi.nlm.nih.gov/pmc/articles/PMC126594/?tool=pubmed
>
> Chromosomal Integration via Homologous recombination of S.
> thermophilus (old name?)
> http://jb.asm.org/content/175/14/4315.short
>
> This paper uses S. thermophilus, but is a bit hard to read
> http://jb.asm.org/content/192/5/1444.short
>
> But it quotes this paper "Efficient insertional mutagenesis in
> lactococci and other gram-positive bacteria.":
> http://www.ncbi.nlm.nih.gov/pubmed/8550537
>
> And another just for kicks "Increasing competence in the genus Streptococcus"
> http://www.micab.umn.edu/courses/8002/Havarstein.pdf
>
>
> I think I have access to all these papers, if others don't let me know
> and I'll post them online somewhere.
>
So with this information of how to get the DNA in and stable... now it
seems like the luminescence or fluorescence system needs to be focused
on.... getting enough precursors so the light is bright enough. Can
heat shock proteins repress enzymes, or only gene expression? (i.e.
the light system is present but not functional, then when they're heat
shocked the enzymes go to work chewing up all the built-up reagent)
--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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