A little off topic but how long does it take to use directed evolution to create extremophiles?
for example if you were to set up some broth with ecoli or similar and heat to the point where 99.9% of the bacteria die.... then culture then heat again.
for example if you were to set up some broth with ecoli or similar and heat to the point where 99.9% of the bacteria die.... then culture then heat again.
and so on for a few thousand generations.
or do a similar thing with spores, use pressure cookers on low to select for the most resistant spores generation after generation.
are there any really hard limits or could someone create a headache for biologists everywhere by breeding organisms which can't be killed in a simple pressure cooker.
or do a similar thing with spores, use pressure cookers on low to select for the most resistant spores generation after generation.
are there any really hard limits or could someone create a headache for biologists everywhere by breeding organisms which can't be killed in a simple pressure cooker.
On Wed, May 30, 2012 at 8:08 AM, Nathan McCorkle <nmz787@gmail.com> wrote:
U.S.-based retailer:
http://www.sciplus.com/category.cfm/subsection/4
also check ebay.
you don't need a $400 camera for visualizing glowing colonies, a cell
phone camera or better works fine
the best HEPA environment is in a laminar flow hood, where the stuff
inside is protected from you, and you're protected from the stuff
inside. The next best thing is a positive pressure box, which can
range from an open faced box where a vertical side is open and the
opposite side is composed of a HEPA filter (24 inch * 24 inch at
least) with a squirrel cage fan pushing air through. The last
box/enclosure option is pretty clunky and a pain to use, its a
still-air glove box, made of a wal-mart type clear plastic storage bin
with arm holes cut in the side, this presents a real problem
transferring things from outside to the inside.
you could also try the build in the attached PDF
On Tue, May 29, 2012 at 10:49 PM, QDragon Leet <dr.qdragon@gmail.com> wrote:
> sorry 1 more thing, what's a good way to jury rig an incubator?
>
> here's my list
> again https://docs.google.com/spreadsheet/ccc?key=0AlRCiEBbcYRjdFJQTFJsQ21QNWhZMTlDZkY2R25oeVE#gid=0
>
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Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics--
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