Re: [DIYbio] Re: Miniprep without EDTA

So, the values are:

For the 209.39 ng/uL plasmid  the 260:280 ratio is 1.46
176.51 ng/uL   -> 1.91
54.17 ng/uL  -> 1.74



I think 1.91 is the best? Should it be high or low? ;)





Am Sonntag, 27. Mai 2012 17:54:23 UTC+2 schrieb Mega:

Hello @all,

I was wondering if I could do a dirty miniprep without EDTA.

What I have: weak centrifuge, SLS (sodium lauryl sulfate), NaOH, vinegar, ethanol (I have access to both wodka and pure ethanol), water.


Can you do a mp with this limited resources?
What I want: Some ~60 to 70 % plasmids, some remaining proteins, RNA, mabe some fragments of chromosomal DNA.


I think with this percentage you can still do a transformation with E.Coli? Because Proteins will either be used or digested, RNA my do it's job inside the cell, chromosomal DNA has no origin of replication.

Only the plasmids will be able to replicate and thus you will get some transformants. These you select with ampicillin anyway. (Clearly, you won't get so much transformants, but who cares? )

Will DNAses inside the solution possibly destroy the plasmids??

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