Re: [DIYbio] Re: Bacteria Lava-Lamp

A synthetic luxCDABE gene cluster optimized for expression in high-GC bacteria
http://nar.oxfordjournals.org/content/35/6/e46.abstract

On Wed, Aug 1, 2012 at 5:22 AM, Nathan McCorkle <nmz787@gmail.com> wrote:
> Expression of the Photorhabdus luminescens lux genes (luxA, B, C, D,
> and E) in Saccharomyces cerevisiae.
> http://www.ncbi.nlm.nih.gov/pubmed/14654435
>
> (I can access the full article, so I think it's open-access)
>
> On Tue, Jul 31, 2012 at 11:25 PM, Chowe <c9howe@yahoo.com> wrote:
>> Im sorry, I do have the plasmid map. It seems that he was referring to the
>> attached document he had sent in the email but I never saw it. I have
>> attached the plasmid map to my post.
>>
>>
>> On Tuesday, July 31, 2012 11:19:26 PM UTC-4, Chowe wrote:
>>>
>>> That is great to hear! I have also plated some glowing cells on amp plates
>>> (my first at home experiment) and I finally got them to glow. It was a great
>>> feeling when I first saw them. Now If I were to put these plates in the
>>> freezer to keep for a while, how long do you think I could save them for
>>> later use?
>>>
>>> Also, I got an email back from the supplier of the gram-positive glowing
>>> plasmid and they said that there is information on the plasmid map on the
>>> site, he wasn't too clear about it. He also added that it is about $561 US
>>> for the plasmid, not very cheap.
>>>
>>> On Monday, July 30, 2012 11:18:48 AM UTC-4, Mega wrote:
>>>>
>>>> Just wanted to tell,
>>>>
>>>> I plated the glowing colis (from my bio fridge) on new plates, and they
>>>> were much brighter than the original ones. Maybe because of less satellite
>>>> colonies because of fresh amp ?
>>>> They were even so bright that you could see their glowing in a semi-dark
>>>> room (10 o clock PM, streetlights illuminating the room weakly) without
>>>> adapting your eyes to it.
>>>>
>>>>
>>>>
>>>>
>>>> Am Sonntag, 22. Juli 2012 01:15:33 UTC+2 schrieb Chowe:
>>>>>
>>>>> Hello! Im very new to DIYbio and I am looking to do an exciting first
>>>>> project. I work in a genetics lab so I have experience with most techniques.
>>>>> I have had many ideas for projects, that I happily found out most people
>>>>> have had the same ones too (glowing plants, glowing yogurt) but I thought a
>>>>> lava lamp would be the simplest way. I am wondering how you guys would go
>>>>> about completing this. I saw that cambridge igem team made a lava-lamp, seen
>>>>> here http://www.youtube.com/watch?v=tUFscEVK5Ks I would like to make
>>>>> something just like that. I would prefer if I didn't have to induce it or if
>>>>> I did it would be through oxygen like the cambridge lava-lamp. It would be
>>>>> cool to have in my room to light it up at night. As with the glowing plants
>>>>> and yogurt they seem to be a lot harder and it seems like not many people
>>>>> have been successful so I wanted to choose a project that I would be more
>>>>> likely to succed. Thanks!
>>>>>
>>>>> Corey
>>
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>>
>
>
>
> --
> Nathan McCorkle
> Rochester Institute of Technology
> College of Science, Biotechnology/Bioinformatics



--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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