Re: [DIYbio] Re: Bacteria Lava-Lamp

On Sun, Jul 22, 2012 at 4:43 AM, Mega <masterstorm123@gmail.com> wrote:
> Hey, I've done something like this...
>
> Took the pVIB plasmid (no need for a specific substrate! just oxygen +
> glucose) in E.Coli and put them into a marmelade jar containing LB medium
> (without ampicillin).
>
> In the dark it was glowing much brighter than a petri dish with those
> bacteria (obviously).
> It stopped glowing after a while (I think one week) perhaps because they
> have consumed all the sugar, or too much endotoxins?)
>
> The best approach however would be to use B.Subtilis. They don't produce
> Endotoxins afaik. And they make spores, so you can just let them run out of
> sugar, wait for month, put sugar into it, and they'll grow and glow again!
> And, of course, bacteria will lose those plasmids after some time. Best bet
> for an eternally glowing lamp is that you insert it into their chromosome.
> (Wanna do this ;) ) And cut out the marker gene (or take a marker that's
> not a clinical antibiotic; such as heavy metal resistance?), to avoid making
> resistant germs...
>
>

A suite of recombinant luminescent bacterial strains for the
quantification of bioavailable heavy metals and toxicity testing
Angela Ivask*, Taisia Rõlova and Anne Kahru

http://www.biomedcentral.com/content/pdf/1472-6750-9-41.pdf


--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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