How about a stir-bar with a mirror or prism on it, and the light source and sensor on the outside of the flask? The mirror could alternatively be at the bottom of the flask, and the light and sensor be at the top, in the stopper.
On Thursday, February 12, 2009 10:53:56 AM UTC-5, Tom Knight wrote:
The Klett is great, but what the world really needs is a high tech--
stir-bar, able to be autoclaved, which can measure OD while a culture
is growing and report out to a readout device. There is no reason that
this cannot be made, and made cheaply. The world would beat a path to
your door, similar to the rapid adoption of the Nanodrop spec. Power
can come from the magnetic field and liquid drag (you don't need much)
and I/O through an IR path, e.g.
Let's not concentrate on making duplicates of old technology; let's
make something better.
On Feb 12, 2009, at 1:31 AM, Douglas Ridgway wrote:
>
> On Wed, Feb 11, 2009 at 12:16 PM, Dan <dan.h...@gmail.com> wrote:
>
>> The point here is if you want to do cell titers, forget OD as a fixed
>> unit of measure and just calibrate it with a cell viability curve and
>> go from there.
>
> There's also the Klett colorimeter, which measures transmission of a
> filtered light (not single wavelength, like OD). Klett is routinely
> measured straight through a culture tube, avoiding the need to sample
> your culture. The one I've used looks like it was built pre-1950, but
> something similar might be an easy DIY build.
>
> >
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