A few important features and scenarios for a vector to work in another host:
1. Proper promoter seq on DNA where the host transcription machinery can bind.
2. Proper 5' untranslated region seq on the mRNA for the mRNA to be translated. (not mentioning codon frequency, rare codons)
3. Further for the vector to replicate in another host it should either have its own replication machinery or else be equipped (For ex. the origin of replication seq in the vector should be same as that in the host) to use the hosts replication machinery.
4. Also replication alone is not enough, the vector needs to partition properly along with the daughter cells of the host such that there are no daughter cells without that vector.
5. This scenario is further complicated by the fact that other naturally occurring plasmids in the new host can out compete ur vector.
On Thursday, 25 October 2012 20:45:10 UTC+2, Chowe wrote:
Hello all,I have a very basic understanding of genetic engineering, I understand how genes are extracted and transferred and expressed. My question is what is the barrier between expressing genes from one organism into another? From my understanding, the main problem in expressing the lux operon in plants is due to the genes originally being expressed in bacteria. But I have also read about bacteria genes being expressed in mammalian cells. Do all genes need to be codon optimized for any chance of being expressed? For example I have a pVIB vector, can I only express that vector in bacteria? Or can I express that vector in fruit flies as well? Lastly, what makes a plasmid backbone different from species to species that I can only express it in that specific species (like expressing a bacteria vector in a mammal cell)? ThanksCorey
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/ngtwrXrUyScJ.
For more options, visit https://groups.google.com/groups/opt_out.
0 comments:
Post a Comment