On Fri, Dec 7, 2012 at 3:43 AM, Cathal Garvey <cathalgarvey@gmail.com> wrote:
Hey Matt,
This is late in coming, but while continuing troubleshooting I looked up
the Kan-resistance gene in pUC57-Kan to determine the mechanism of
resistance.
Turns out that it's a phosphotransferase rather than a resistant
ribosome; so, the resistance gene doesn't confer immunity, it instead
degrades the antibiotic, like Ampicillin.
Still lower chances of satellite colonies or contamination of course, as
it kills non-resistant cells early in culture.
Still though; there are resistant ribosomes out there, right? So why
aren't they used for resistance genes instead? Ribosomal genes shouldn't
be very large, and conferring resistance rather than destroying
antibiotic could help prevent plasmid loss in later phase cultures?
Seems like resistant ribosomes would be worse for overall expression levels, since there'd be competing ribosomes, some of which would be generating truncated proteins, effectively wasting cellular resources
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.
0 comments:
Post a Comment