I just got a ad-email from GeneArt telling me that in my country there's special condition for academic labs... only 25 cents per base pair but limited in time. If I knew it would work, I'd have it synthesized immediately with the super-strong plant promoter and optimized renilla luciferase.
(In total that would be 625€ which I'd consider acceptable prize for such a project)
But - as said, if there's low (or no) chance that it works, it'd be pointless waste of rare money.
On Sun, Dec 30, 2012 at 5:09 PM, Jordan Miller <jrdnmlr@gmail.com> wrote:
no I'm not sure. but yes it does seem too good to be true.if you get it to work let us know!luminometer has the highest signal to noise of any assay so I'm positive it didn't work how we did it.
jordan--Jordan:we did the point mutation on eGFP optimized for bacterial expression with the induced vs. non-induced controls and coelenterazine as the positive control with purified Gaussia luciferase from a mammalian expression system. did not work as expected in our hands.But, are you *sure* that with wild-type GFP there would also not be luciferin production?It's worth too much to not trying it.... It would be the first (and only) eukaryotic light generating pathway, consisting of only two (!) genes... Too good to be true???-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
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