Remember, you're not reading a scientific paper, you're reading a *patent*. Patents are not written to be legible, but to be legally defensible. Patent lawyers play by entirely different rules, which entirely too often seem completely nonsensical.
On Saturday, December 29, 2012 6:19:58 AM UTC-5, Mega wrote:
Honestly, this--Suitably, the regulatory element may be a promoter. Suitable promoter elements include a promoter activated by heavy metal (e.g. the one described in Freedman, et el. J. Biological Chemistry, 268: 2554, 1993, incorporated herein by reference); a P 450 promoter (e.g. the cytochrome P 450); or a promoter for a stress protein, (e.g., described in Stringham, et. el., Molecular Biology of the Cell, 3: 221, 1992), one of said stress proteins being a heat-shock protein. Other suitable promoters include that of the arabinose operon (phi 80 dara) or the colicin E1, galactose, alkaline phosphatase or tryptophan operons. Similarly the ADH system may be employed to provide expression in yeast. Alternatively, the regulatory element may be an enhancer.
The regulatory control sequences are operatively linked with the polypeptide comprising one or more sequences of nucleotide bases collectively encoding an amino acid sequence of a pre-coelenterazine peptide; i.e., the regulatory control sequences are placed on the polynucleotide at a distance 5' of the one or more sequences suitable to enable expression of the sequences.
Polynucleotides which bear one or more of such regulatory control sequences may be used in transforming organisms, as when suitably the polynucleotide is included in an expression vector. The regulatory control sequences are selected for compatibility with the organism into which the polynucleotide is to be incorporated by transformation, i.e., the regulatory control sequences are those which may be recognized by the transformed organism or cell and which will aid in controlling the expression of said polynucleotide in the transformed organism.
Thus when the organism to be transformed is E. coli, the regulatory control sequence may be a promoter (e.g., the T7, the SP6 or lac promoter); or transcription initiation sequences for ribosome binding (e.g. the Shine-Delgarno sequence and the start codon AUG). When the organism to be transformed is eucaryotic, the regulatory control sequences may include a heterologous or homologous promoter for RNA polymerase II and/or a start codon AUG. For example, when the target of transformation is a mammalian cell, the regulatory control sequence may be a promoter (e.g. the SP 40 or the bovine papilloma virus promoter). Suitable regulatory control sequences for use in other microbe, or in animal, or plant cells may be selected according to criteria well known to persons having skill in the art. All of these regulatory control sequences may be obtained commercially (individually or incorporated into a vector) or assembled by methods well known in the art.
Where the polynucleotide carries one or more appropriate regulatory control sequences, there may further be present one or more further sequences of bases which collectively confer resistance to an antibiotic when the polynucleotide is expressed in an organism. Such genes for antibiotic resistance are desirable components for expression vectors since they facilitate identification of transformed cells grown in the presence of antibiotics, and exert a continual pressure on the transformed organisms to retain and express the expression vectors.
could have been left out. It should have been left out, to make it understandable,
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