Well I'm confused now but.
Let's say we have a piece of filter paper 10cm long, 1 cm wide, and 0.1 cm thick.
Is it wrong to think it could handle similar voltages and currents that an agarose gel could?
Say it was running at 100V and 0.050A. Could you then use V=IR to calculate the resistance of the buffer soaked paper would be 2000 Ohms?
Or, via P=IV, P = (0.050A) x (100V) = 5W
And 5W = 5 J/s * 600 seconds (10 minute run) = 3000 Joules
That's a lot of energy to put through a buffer soaked piece of paper soaked between two glass slides, no? Though I guess if it's submerged in the giant buffer tank it has a heat sink sort of.
I havn't done good ol' electricity basics in a while so I'm probably wrong in the 2000 Ohm part if not everywhere.
What I'm wondering is...WITHOUT trial and error, could you calculate the "perfect" conditions to run an agarose gel under - meaning voltage + current assuming a tris buffer?
Likewise, could we calculate the optimal conditions to run an electrophoresis 10,1,.1 cm thick piece of paper with tris buffer?
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.
0 comments:
Post a Comment