exhaustion of bufferWhat about using less concentrated buffer? How low can you get down? Instead of 1* maybe 0,7 would do?
long run times
What about including peltier chips or putting the chamber in a freezer with a fan? These would cool the buffer and gel and thus you could apply more voltage. Double voltage means half run time...
On Wednesday, May 11, 2011 9:05:20 PM UTC+2, Nathan McCorkle wrote:
Does anyone know of ways other than electrophoresis of DNA to
separate? The problems with long run times and exhaustion of buffer
seem like they could be alleviated... but how? Maybe something like
flow cytometry, but in a much smaller capillary? Maybe the DNA could
be ligated to something like sialyl-lewis-X, and the capillary coated
in CD62P (protein that binds SLX) to slow down the DNA during its
flow... then detection could proceed with UV spectroscopy (the
capillary would need to be very thin to allow differentiation of
single/few molecules)What other ideas do people have?
--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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