Re: [DIYbio] Re: BioPrinter won 1st Prize in Instructables Design Competition!



On Fri, Mar 1, 2013 at 4:18 AM, Nathan McCorkle <nmz787@gmail.com> wrote:

What would you propose for a pulse-free pumping solution in the uL/nL
range? I know a pump can handle those volumes, but what kind of tip
would let go of uL or nL droplets? The printer cartridge ejects pL
droplets.


First explain why you need nL or pL droplets.  Yeah I know it's all the rage at MIT to constantly make everything smaller for apparently little purpose other than to claim it's novel and get a mention in Wired.   From the instructable directions, there's paragraphs explaining that using a special inkjet head with fatter nozzles is needed to overcome problems anyway, i.e. 'The problem with inkjets is that they're resolution is too *high* and we need lower resolution' (i.e. fat pipes).   For a petri dish pL or nL is not needed.. for microarray they are not needed..  Even if you're going to try microfluidics with pL volumes you'll run into other problems (how about evaporation?) so as mentioned before, perhaps the best hack right now is milli-fluidics not micro.    For microliters the commercial comparison is Biotek microflo (likely others, that's just one I evaulated) and it uses plastic tips and tubes to dispense 0.5 uL - 3,000 uL properly.


Volume Range 500 nL - 3,000 µL/well
Selectable in 1 µL increments


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## Jonathan Cline
## jcline@ieee.org
## Mobile: +1-805-617-0223
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