[DIYbio] Re: Non-Platinum Electrophoresis Electrodes That Don't Degrade

I would not call one dollar per cm "pretty expensive". That's what, $20-40 per gel box? How does that compare to the power supply you're using to run the electrophoresis?

I've used stainless steel wire without much noticeable corrosion during a single run. But yeah, I would expect you'll need to replace the wire every couple of gels at least. If your gel box is designed such that it only takes a few minutes to swap out fresh stainless steel wires for every gel, that could defintely be an option.

Anyone see any side effects you might get from having some additional iron or chromium ions in the buffer, from corroding stainless steel electrodes?

Patrik


On Sunday, May 26, 2013 11:29:19 AM UTC-7, Josiah Zayner wrote:

I have been testing out a bunch of different metals and they either oxidize to death on the minutes timescale or in the case of stainless steel turn the buffer orange/brown.

Platinum wire is pretty expensive ~$1.00 cm^-1. Has anyone had luck with a cheaper metal at 100V or greater with TAE?

I have tried:

Nickel

Stainless Steel 430

Tinned Copper

Thinking of trying Titanium or Nickel Titanium (<= $4 meter^-1). Anyone ever tried these?

Thanks,

     Josiah Zayner

http://DoItOurselfScience.blogspot.com 

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