Re: [DIYbio] Non-Platinum Electrophoresis Electrodes That Don't Degrade

The graphite rod with a compressed o-ring is a good idea I think.
We're planning to build gel boxes here in Portland soon, I'm scraping
together ideas for what to try out now. Vacuum forming came to my
mind, but if the plastic is so flimsy how would you compress the
o-ring? I'm thinking of trying HDPE from Gallon sized milk jugs, and a
youtube video recommended polystyrene disposable kitchen/picnic
plates.

But where to get cheap graphite rods? Amazon.com sellers want around
$1/inch... seems a pencil is about 20-30X cheaper. Maybe firing
pencils would allow extraction, or is pencil graphite not actually
lead (like how they're called lead pencils)?

On Mon, Jun 3, 2013 at 3:17 PM, John Griessen <john@industromatic.com> wrote:
> On 05/26/2013 10:34 PM, Josiah Zayner wrote:
>>
>> Graphite rods look like an ok solution but seem complicated
>> to attach and use
>
>
> How about O-ring seals in a gel box wall. Then connectors and metals
> are all outside the box.  Perhaps a water tight cord grip for electric power
> cords
> would be an expedient DIY method of constructing such a gel box feed through
> for
> a graphite rod.  The other end of the rod could go to a hole that only goes
> half
> way through the gel box to support the rod end so it's not along lever
> that could let it get broken easily.  For an open hardware product, a simple
> turned shape attached by screws could clamp against an O-ring in its
> groove to seal the rod.
>
> On 05/26/2013 11:44 PM, Dakota Hamill wrote:wouldn't it be just as, if
>
>> not more cost effective to go with
>> platinum wire?
>>
>
> Sure, but it only benefits one at a time DIY expedience.  More effort is
> worth
> it if you can develop open hardware to share, especially if it results
> in ease of use, long useful life, cleanable, rebuildable, etc.
>
> For circuit printing, there are carbon inks that cure with heat.
> What if one wall of a gel box was printed with in a big rectangle
> of conductive carbon "ink" that goes above the gel height?
> Wonder if it would last like pure carbon rods?
>
>
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--
-Nathan

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