Just noticed, it would also be possible to change the second amino acid from isoleucine to valin/alanine/Glutamic acid/Glycine/aspartic acid to create the same restriction site.
Just had a look at valine, and it looks quite the same :D
On Monday, October 7, 2013 4:47:03 PM UTC+2, Mega [Andreas Stuermer] wrote:
-- Just had a look at valine, and it looks quite the same :D
On Monday, October 7, 2013 4:47:03 PM UTC+2, Mega [Andreas Stuermer] wrote:
Hi everyone!
For expression of a protein I would need to make it fit to the RBS of the vector.
Is it ok, if I attach ATG-GGC (Methionine and Glycine) to the N-Terminus?
So the fusion protein would be Met-Gly-Met-rest of Protein
Is there a guarantee that the protein still works then?
Best,
Andreas
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