Re: [DIYbio] Expression Problem - fusion protein

On Mon, Oct 7, 2013 at 1:34 PM, Richard Yu <ryu@radiantgenomics.com> wrote:
> Yep, the second residue determines cleavage efficiency, and then also
> influences protein lifespan (N-end rule).

Huh, that's a new thing to look up I hadn't heard of.


> N end rule in e coli, from Varshavsky's 1991 Science paper:
> Amino-terminal arginine, lysine, leucine, phenylalanine, tyrosine, and
> tryptophan confer 2-minute half-lives on a test protein; the other
> amino-terminal residues confer greater than 10-hour half-lives on the same
> protein.

Very very interesting, in all the cell and molbio classes I've had, I
don't remember hearing about this.

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