There was a paper, which descirbed the construction of a grm-positive lux vector and even revealed the sequence...
Can't find the article anymore, but if I can send you for future projects...
Similar ones...
There was also an old one for B. Subtilis, which glowed noticable by naked eye when carriying the high-copy number plasmid. But when a single-copy was integrated into the genome, it glowed too weak to see.
On Sun, Oct 6, 2013 at 12:31 PM, Andreas Sturm <masterstorm123@gmail.com> wrote:
As for pVIB, in E. Coli it works just awesome.
Ah, lactobacillus is gram positive... So pVIB won't work well with that. Once there was research done which added gram positive RBS and a bacillus promoter to the lux operon.
I believe your idea could make GMOs more acceptable.People see your yoghurt and it says "makes you live longer and healthier" and "contains GMO derived products". Then they have to choose. And even the most (pseudo-)environmentalist wants to live a longer healthier life...
So, many people will choose GMO if it brings health benefits (instead of making it insecticide producing)On Sun, Oct 6, 2013 at 9:12 AM, Nathan McCorkle <nmz787@gmail.com> wrote:
On Sat, Oct 5, 2013 at 4:29 PM, Richard Yu <ryu@radiantgenomics.com> wrote:
> We're looking into what's the best way to release something live- live
> yogurt, or a kit that you can transform in a lab (lacto + plasmids), or
> something else.
> I amWith provide-your-own yogurt and centrifuge, providing a plasmid could
> intrigued by the idea of plasmids as a perk for a pledge; I'll definitely
> pitch that at our next meeting.
be a proof-of-concept kit! I've been working on building an
electroporator for a while, last used e.coli and a grill lighter piezo
unsuccessfully, but there are a bunch of papers on l. delbrueckii
getting zapped that I'd be excited to try! Do you have any of the
shuttle vectors now?
None of them really mention using milk agar though, mostly MRS media
which is somewhat expensive at $160 for 500g powder.
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