There is already a patent for this process: http://www.google.com/patents/WO2006124999A3?cl=en
Though I don't know how much that matters. The glowing plant project was selling someone else's patented idea.
Resveratrol from what I see can be purchased for really cheap 15-30g for $11 or so. How does your system make it any better than just me adding resveratrol to my yogurt?
What is the estimate in the amount of resveratrol that can be produced in say 200mL of yogurt? The paper you put in your Indiegogo says 16mg/L in E. coli(probably much lower for a wildtype Lacto). That is more than wine but at that amount(3mg/200mL) I could pay $11 and have >20L of resveratrol laden yogurt.
Your Indiegogo says 10x the amount found in wine. From what the literature says, taking a median value, there is currently around a 4 fold difference (16mg/L, E. coli and 1-7mg/L Wine). How do you plan to come up with the other ~6 fold? Or are you using the lower estimates of resveratrol in glasses of wine? In the paper you cite on your Indiegogo for increased resveratrol production they are engineering yeast and though they say they have a 2.44 fold higher resveratrol production it is still only ~3mg/L total. The other papers I have seen on higher resveratrol production are in yeast also.
So do you have any idea how you will increase this production? Or if the system will even work in Lacto?
On Sunday, October 6, 2013 4:43:27 PM UTC-5, Richard Yu wrote:
Hi Andreas,I certainly hope that it helps, at the very least, create some constructive dialogue and perhaps open some previously closed minds.I want to see realized the potential of this for enabling the poor to make their own meds, meds that are not being produced for the usual economic reasons. I hope that that is something most people can consider a worthy goal, despite the large amount of work it will take to get there safely.As for promoters, we're planning on using native constiutive/strong Lactobacillus promoters, might as well stay food-grade an not go heterologous. We're also using only genes from red grapes. Addresses the potential "allergy" issue of using insect (or other foreign) proteins.A humble request:If you guys like what you see, please follow/tweet about us @yivivoyogurt and like our page on Facebook https://www.facebook.com/yovivoyogurt ? WE're in the early stages of our campaign and could really use the early momentum and support! (Contributions would be awesome, too! I'm working on the live/kit thing ASAP!)Thanks all! I really appreciating the genuine psyche and interest in DIYbio. Hope to keep the conversation going.Rich
On Sunday, October 6, 2013 3:31:13 AM UTC-7, Mega [Andreas Stuermer] wrote.[...]I believe your idea could make GMOs more acceptable.People see your yoghurt and it says "makes you live longer and healthier" and "contains GMO derived products". Then they have to choose. And even the most (pseudo-)environmentalist wants to live a longer healthier life...
So, many people will choose GMO if it brings health benefits (instead of making it insecticide producing)[...]
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