Make your own Elliker medium! Here's the broth composition that I got out of the Journal of Dairy Sciences from 1956:
Tryptone 2%
Yeast extract 0.5%
Gelatin .25%
Dextrose .5%
Lactose .5%
Sucrose .5%
Sodium chloride .4%
Sodium acetate .15%
Ascorbic acid .05%
All percentages are w/v; if you're going to pour plates, add 1.5% agar. See also http://www.jsunitech.com/product/culture/manual/Elliker%20Broth.pdf, which gives measurements in SI units (5g yeast extract, 2.5g gelatin, &c), and swaps in casein peptone as a replacement for tryptone.
Cathal, whatever happened to your casein peptone protocol? I can't find it on your blog anymore :(
According to http://www.scribd.com/doc/24840826/2010-Lactic-Acid-Bacteria-Potential-for-Control-of-Mould-Growth-And, Elliker medium is actually better than MRS broth for production of antifungal compounds, for what that's worth.
Cheers,
--mlp
On Sun, Oct 6, 2013 at 9:12 AM, Nathan McCorkle <nmz787@gmail.com> wrote:
On Sat, Oct 5, 2013 at 4:29 PM, Richard Yu <ryu@radiantgenomics.com> wrote:
> We're looking into what's the best way to release something live- live
> yogurt, or a kit that you can transform in a lab (lacto + plasmids), or
> something else.
> I amWith provide-your-own yogurt and centrifuge, providing a plasmid could
> intrigued by the idea of plasmids as a perk for a pledge; I'll definitely
> pitch that at our next meeting.
be a proof-of-concept kit! I've been working on building an
electroporator for a while, last used e.coli and a grill lighter piezo
unsuccessfully, but there are a bunch of papers on l. delbrueckii
getting zapped that I'd be excited to try! Do you have any of the
shuttle vectors now?
None of them really mention using milk agar though, mostly MRS media
which is somewhat expensive at $160 for 500g powder.
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