Hi K,
I don't know at this point how easy or hard a tet switch will be; I don't have time to do the research as I'm up in Dublin for a few meetings but will catch up when I'm back in Cork.
The crowdfunding campaign is my attempt to get funding from the people who'll use and reshape the plasmid the most. If any iGEM teams happen to have spare cash lying around (?) I'd be very grateful for their support, but frankly I doubt it; I imagine they work to a budget and can't spare the excess.
As far as biobricking the individual plasmid components, that's not really practical while making the multiple-hacking-site biobrick compatible, and it adds overhead that's unnecessary. If someone wants to PCR out the Colicin cluster with biobrick'd primers, that's fine. As per Andreas' great suggestion that I make the individual components of the plasmid easily extractable, I'm hoping to include useful priming sites around each part which would facilitate such an efffort.
I can't let feature-creep bloat this too much, though. I want it to be 'hackable' but that has to be achievable within spec! :)
I agree that there should be a toxin switch if it is simple for you to make.Also, I had another thought for your campaign. Perhaps make the colicin part biobrick compatible. Try and get iGem teams to fund your project as well, since there is quite a few of them and a self selection method would allow for some awesome biological switches, just what they are looking for.-Koeng
On Thursday, January 30, 2014 12:57:31 AM UTC-8, Cathal Garvey (Phone) wrote:Colon is basically anaerobic or microaerobic, you'd get no glow. If anything, the luciferase would eat up what oxygen remained!"Mega [Andreas Stuermer]" <masters...@gmail.com> wrote:This night I was just thinking about the following:What if you put the lux operon into it and somehow get the bacteria in your gut? And, if they would be able to stay in thereThen your intestines would be weakly glowing (but lower oxygen levels would likely limit this)The blue light would help stimulate DNA repair, so colon cancer rates may be drastically lowered?I assume the light induced DNA repair mechanism (via p53) is still present in human cells.
--
Sent from my Android device with K-9 Mail. Please excuse my brevity.
--
Sent from my Android device with K-9 Mail. Please excuse my brevity.
0 comments:
Post a Comment