Ok next week I will try snap freezing the cells and then placing them into the -20
-- One thing though: Yeast comp cells are supposed to be frozen very slowly in order to preserve them... Like if you want to get cells you put them in a styrofoam box then place it in the -80. Wouldn't this allow more time for ice crystal formation?
On Friday, June 27, 2014 4:33:29 PM UTC-7, John Griessen wrote:
On Friday, June 27, 2014 4:33:29 PM UTC-7, John Griessen wrote:
On 06/27/2014 06:06 PM, Koeng wrote:
> Has anyone tried this?
No, but I thought it was the rapidity of freezing that avoided
cells being stabbed/deformed by ice crystals by way of smaller ice crystals
in rapid freezing... If so, freezing in your -80, or liquid N2, then storing in -20 might get results.
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