Well the Idaho Tech halogen light / vortex air thermal cycler which was the first one i got off ebay has one major advantage over any peltier device I've used - it saves a boatload of time. The ramp rates are much faster, especially the decreasing, as a vent is just flips open and hot air pours out. It really cuts the PCR program almost in half on time, though I should actually measure it, but it generally finishes a good 30-45 minutes before a peltier device doing a 30x program, which normally takes ~1.5 hours.
It can't hold 96 samples, and requires oil due to no heated lid, but it's worked before and the time it saves more than makes up for those other disadvantages for the uses at the moment. Obviously being able to run a lot of samples in parallel with a temperature gradient for annealing temp without oil is where the peltier machine is better but, it's also a new machine. There are plenty of old peltier based devices without heated lids with crappy ramp rates, and for that I prefer the lightbulb machine.
On Mon, Jun 30, 2014 at 11:48 AM, John Griessen <john@industromatic.com> wrote:
On 06/30/2014 06:05 AM, Cathal Garvey wrote:Are there some good research reasons to go for more delta T rate?
t's rare that you'll need decreasing ramp rates faster than 1C, in any
case. Have you a special experiment in mind?
My stirred air cooled PCR cycler concept is likely to do more
when I get around to doing it and testing it. And for evenness,
it will be top performing inherently.To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/53B186B8.40606%40industromatic.com.
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