Re: [DIYbio] Relatively safe-to-handle mutagens to expose seeds to?

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Sebastian; would it work if you were to remove the outer coat? How often
does that just kill seeds?

If you removed the coat, possibly even removed one of the seed halves to
expose more of the embryo, you might be able to mutate the embro and
support it through germination with TLC..

Alternatively, don't work with seeds; culture a callus of
undifferentiated cells and expose those to abundant UVC, or grow on a
tobacco-infused agar medium. Callus is already pretty happy to mutate
somatically as it doesn't have the same structural mechanisms used by
plant meristems to exclude mutant cells.

On 25/08/14 21:53, Sebastian Cocioba wrote:
> According to Ames, all mutagens are carcinogens. The UV sterilizers use UVC
> short wave but may not penetrate thick seed coats. Arabidopsis or orchid
> seeds are thin and lack opaque starches so the light may penetrate deeper.
> If you don't want to commit I could run some and show results.
> On Aug 25, 2014 4:35 PM, "Nathan McCorkle" <nmz787@gmail.com> wrote:
>
>> On Mon, Aug 25, 2014 at 1:14 PM, Warren Notes <warrennotes@gmail.com>
>> wrote:
>>> Anyone have any thoughts on UV light as a mutagen?
>>
>> It works for e.coli, though I feel like with a seed you'd not have
>> such luck as the penetration depth is probably pretty low. Google
>> Scholar it up!
>>
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