Sebastian, as a miniPCR team member, can you comment on the lack of documentation for PersonalPCR? At first glance it appears that it was released as a fully functional and open platform, but after actually digging into it it is not only incomplete (microcontroller layout is unfinished and nonfunctional, for example) and the source code is in disarray. I assume miniPCR finished the hardware design and software implementation, but is there any future for personalPCR that will allow it to be built as a functional thermocycler?
-- I am interested in seeing more of your design in the real world, and would very much like to build one myself. I actually have all the components on hand, as well as a shop to build one in. At the moment I am essentially forced to remake the design from the ground up -- which is fine, but not ideal for either the DIYbio community or the claims of miniPCR being an open platform.
On Friday, September 5, 2014 9:32:49 PM UTC-4, Sebastian Kraves wrote:
On Friday, September 5, 2014 9:32:49 PM UTC-4, Sebastian Kraves wrote:
Hey guys this is Sebastian. I am part of miniPCR.Dakota -- you're right. Zeke (Ezequiel Alvarez Saavedra) carried Personal forward into miniPCR. You should definitely visit us and have a look at the mini. We'd be happy to have you over.Jarle -- it's good that there are different options. Different PCR machines will be better suited for different users. miniPCR cooling is not Peltier but convective. This helps reduce the machine size to a format that's easily moved around and shared across labs, or tucked away when not in use. Performance is similar to any high-end PCR instrument. we spent 6 months post-development validating and refining at top-notch academic labs (Harvard, MIT, UMass, etc.). It also has a software interface geared towards simplicity. For example, you can program miniPCR off your Android phone.For us, one of the key drivers was to sell a product that's ready to go out of the box and has been fully QC'ed. Happy to chat if you have questions.Cheers,Sebastian
On Friday, July 25, 2014 6:11:28 AM UTC-4, jarlemag wrote:In the last few years, several low cost (in relative terms) PCR thermocycler designs have become available for purchase. I only have personal experience with one of these, OpenPCR. However, other alternatives include PersonalPCR (http://personalpcr.com/) and miniPCR (http://minipcr.com/). Does anyone have experience using more than one "low-cost" PCR design? If so, can you tell a little about the differences, advantages and drawbacks of each? As far as I know, OpenPCR is the only one to use a peltier element for cooling. Do you consider this an important feature?I also noticed that Ezequiel Alvarez-Saavedra is on the team of both PersonalPCR and miniPCR. Does anyone know the signficance of this/the relation between these two? Is the design very similar?Lastly: Does anyone know the status of the LavaAmp project? There have been no official updates for a long time. I vaguely remember reading somewhere that it is still under development and a reason being given for the lack of updates, but I can't recall the details. Does anyone know anything?Best regards,Jarle Pahr
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