Re: [DIYbio] Phage assisted DNA distribution

On Sun, Sep 7, 2014 at 11:45 AM, Koeng <koeng101@gmail.com> wrote:
> Disadvantages
> Possibly mutates quicker? (single stranded intermediate?)
> Size limitation*
> Lab receiving has to have F plasmid E coli
> Contaimination issues***
> (There would probably be a ton of contaimination since the phage are so
> infectious )

Yeah so how do you 'cure' a batch of E.coli of the phage?

I tried looking up some keywords like inhibition and 'cure' but I
didn't get too much:
http://diyhpl.us/~nmz787/pdf/RIFAMYCINS_A_GENERAL_VIEW.pdf

Couldn't get this one:
http://www.sciencedirect.com/science/article/pii/0042682272903947


Seems like having a switch where they won't synthesize, and maybe
another switch for dissolving the strands somehow would be nice. If
they're really so infectious, how do you otherwise normally keep them
under control and having some uncontaminated stock culture?

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