Re: [DIYbio] Phage assisted DNA distribution

On Mon, Sep 8, 2014 at 5:24 AM, Koeng <koeng101@gmail.com> wrote:
> The phage only get synthesized in the presence of M13KO7 and only infect in
> the presence of the F plasmid. The shipped phage won't have either one.
>
> However, you'd have to make sure your stock of F plasmid E coli (SS320)
> don't get contaminated.

So do you transform with F plasmid prior to phage use every time to
prevent contamination of your untransformed stock culture?

Have you come upon any means of curing the culture of the phage? The
rifamycin seems to only stop synthesis, but the paper didn't mention
anything about catabolism/disintegration of the phage particles.


If you don't do this, do you have a lab room that is devoted to phage
work? And have a different room for your stock culture operations?

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