Hello,
Hope you doing well,
Please go through the requirement and let me know on dhruv@riderconsultinginc.com that you are comfortable.
Role : Data Center Project Manager
Location: St. Paul, MN
Duration: 1 year
Description:
Healthcare system has immediate openings for project manager to fill the role of Data Center Enhancement project manager. The project manager will lead the effort to determine current state and proposed state of the Fairview Data Center. This will include documentation of options/costs/etc., to present to senior leadership. Once path forward is determined (option chosen), the project manager will lead the implementation of the Data Center enhancement.
Skills:
· Effectively establishes and maintains working relationships at all levels.
· Excellent analytical and planning skills.
· Demonstrated leadership.
· Excellent communication and customer service skills.
· Demonstrated flexibility and diplomacy.
· Healthcare industry experience preferred.
· Substantial 5-7 years Project Management experience.
Thanks,
Dhruv Soni
Phone : 218-656-0396
Email : Dhruv@riderconsultinginc.com
Gtalk : rider.dhruv1
Hello,
Hope you doing well,
Please go through the requirement and let me know on dhruv@riderconsultinginc.com that you are comfortable.
Role : Front End Lead
Location : NYC,NY
Availability : Immediate
Job Description
Required Skills:
· 8+ years of industry experience, with a minimum of 3 years in user experience design
· A deep understanding on UX design process and activities
· Strong working knowledge on one/many RIA frameworks and JavaScript libraries (Angular JS, Bootstrap, Node.Js, Socket.io)
· EXPERT in JavaScript, HTML5 and CSS
· Understanding of Java MVC frameworks (e.g. Spring MVC), RESTful Services will be a considered an advantage
· Excellent communication and presentation skills
Thanks,
Dhruv Soni
Phone : 218-656-0396
Email : Dhruv@riderconsultinginc.com
Gtalk : rider.dhruv1
Ah Sebastian, you rock. Thanks for the rigorous and methodical write-up. :)
See attached photo for an example of my crappy attempt at making pdms more hydrophilic using a UV sterilizer (the breadbox type). 30 minute exposure on both a piranha-treated microscope slide (15min in a solution of 3:1 h2so4:h2o2 *dangerous, corrosive, always use appropriate PPE*) and a freshly minted pdms chip. I dried the slide with a kimwipe and used scotch tape to remove residual dust from the feature side of the pdms chip. Then I just laid the slide and pdms chip (feature side up) into the chamber and set a timer for 30 minutes. Then quickly place the pdms chip feature side down onto the slide and apply gentle pressure around the features. Be sure to not squash the channels.
PDMS was the knock-off ML Solar brand from eBay. 10:1 mix by weight. Degassed in a desiccation chamber to 25+inHg twice and incubated at 60c for 1hr.
Pdms chip was casted in a plastic Petri dish on a shrunk shrinky dink printed using graffix brand shrinkable sheet (white not clear!). Something funky about the clear version. Sticks to everything when in the oven, got better results with white sheets. Printed with a standard brother monochrome laser printer (toner). Chip design taken from template PowerPoint slide from this site:
http://chemistry.beloit.edu/Edetc/nanolab/shrink/index.html
Its a simple gradient generator. Still not as fluid and wettable as I'd like it to be. I used a straw to mouth-vacuum from the outlet port. Aside from some funky phenomena on the rightmost channel, it did seem to separate decently. I used food coloring as the indicator.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&DOn Sun, Dec 28, 2014 at 8:47 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
From: Nathan McCorkle
Sent: 12/29/2014 3:21 AM
To: diybio
Subject: Re: Microfluidics Chat Thread WAS: [DIYbio] Paper request
> On Sun, Dec 28, 2014 at 8:45 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
>> https://raw.githubusercontent.com/nmz787/microfluidic-cad/master/implicitCAD/output/tobacco_mesophyll_protoplast_fusion_device.jpg
>
> and yeah I know I need to improve the funnel-of-posts angle so the
> edges follow the pitch of the posts, so i.e. cells don't get stuck.
Which is demonstrated on the macroscale with marbles:
https://www.youtube.com/watch?v=03tx4v0i7MA
- Microfluidics Chat Thread WAS: [DIYbio] Paper request - 1 Update
- Yet another paper request - 7 Updates
- saccharomyces cerevisiae potentialities - 2 Updates
<scocioba@gmail.com>: Dec 30 11:29PM -0500
See attached photo for an example of my crappy attempt at making pdms more hydrophilic using a UV sterilizer (the breadbox type). 30 minute exposure on both a piranha-treated microscope slide (15min in a solution of 3:1 h2so4:h2o2 *dangerous, corrosive, always use appropriate PPE*) and a freshly minted pdms chip. I dried the slide with a kimwipe and used scotch tape to remove residual dust from the feature side of the pdms chip. Then I just laid the slide and pdms chip (feature side up) into the chamber and set a timer for 30 minutes. Then quickly place the pdms chip feature side down onto the slide and apply gentle pressure around the features. Be sure to not squash the channels.
PDMS was the knock-off ML Solar brand from eBay. 10:1 mix by weight. Degassed in a desiccation chamber to 25+inHg twice and incubated at 60c for 1hr.
Pdms chip was casted in a plastic Petri dish on a shrunk shrinky dink printed using graffix brand shrinkable sheet (white not clear!). Something funky about the clear version. Sticks to everything when in the oven, got better results with white sheets. Printed with a standard brother monochrome laser printer (toner). Chip design taken from template PowerPoint slide from this site:
http://chemistry.beloit.edu/Edetc/nanolab/shrink/index.html
Its a simple gradient generator. Still not as fluid and wettable as I'd like it to be. I used a straw to mouth-vacuum from the outlet port. Aside from some funky phenomena on the rightmost channel, it did seem to separate decently. I used food coloring as the indicator.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
-----Original Message-----
From: "Nathan McCorkle" <nmz787@gmail.com>
Sent: 12/29/2014 3:21 AM
To: "diybio" <diybio@googlegroups.com>
Subject: Re: Microfluidics Chat Thread WAS: [DIYbio] Paper request
>> https://raw.githubusercontent.com/nmz787/microfluidic-cad/master/implicitCAD/output/tobacco_mesophyll_protoplast_fusion_device.jpg
> and yeah I know I need to improve the funnel-of-posts angle so the
> edges follow the pitch of the posts, so i.e. cells don't get stuck.
Which is demonstrated on the macroscale with marbles:
https://www.youtube.com/watch?v=03tx4v0i7MA
--
-Nathan
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<scocioba@gmail.com>: Dec 30 02:08PM -0500
I'm lounging around at the museum of natural history and during some downtime I stumbled upon yet another paywalled article about plant protoplasts and microfluidics:
Developing microfluidics for rapid protoplasts collection and lysis from plant leaf
http://pin.sagepub.com/content/226/1/15.abstract
This one is interesting since it works with isolation, single cell sieving, and lysis for DNA extraction all in one chip. Would anyone care to help a broke plant biologist this holiday season? Thanks in advance!
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
Tom Knight <tk@mit.edu>: Dec 30 02:14PM -0500
<scocioba@gmail.com>: Dec 30 02:15PM -0500
Great idea. Sure beats spamming the list for my needs.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
-----Original Message-----
From: "Tom Knight" <tk@mit.edu>
Sent: 12/30/2014 2:14 PM
To: "diybio@googlegroups.com" <diybio@googlegroups.com>
Cc: "Thomas Knight" <tk@mit.edu>
Subject: Re: [DIYbio] Yet another paper request
Here you are.
If you want a more predictable set of replies, then I'd recommend the Bioforum group at protocol-online.org. The "Papers" subgroup will find virtually anything, and has people with access to things I can't get. The group is friendly in general.
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Tom Hodder <tom@limepepper.co.uk>: Dec 30 07:34PM
try these 2
http://sci-hub.org/
http://gen.lib.rus.ec/
they are obviously as dodgy as a 3 pound note, but they usually have what
you want and can also be configured to transparently proxy most of the
journals web sites.
Tom Hodder <tom@limepepper.co.uk>: Dec 30 07:35PM
oops. that was meant to be off list. Sorry.
Nathan McCorkle <nmz787@gmail.com>: Dec 30 05:56PM -0800
> Developing microfluidics for rapid protoplasts collection and lysis from
> plant leaf
> http://pin.sagepub.com/content/226/1/15.abstract
This is another really cool design. Lots of great ideas!
Sebastian Cocioba <scocioba@gmail.com>: Dec 30 09:25PM -0500
Yeah the clever cell sorting array is what I'm super curious about. I want
to see if my toner printer can handle such small structures using shrinky
dinks. Just going to overlay my hemocytometer on the chip and make various
posts in a PDMS chip and see what works. Know of any high DPI laser
printers for cheap?
mostromundo <mzuorski@gmail.com>: Dec 29 09:50PM -0800
I work in wine production, we want yeast that will consume all of the
hexose sugars (especially fructose) and will do so while producing more
glycerol and less ethanol. If you can make a fructophilic wine yeast with
below-average ethanol production and acceptable sensory characters, you
will make a lot of winemakers very happy :)
On Friday, December 26, 2014 9:29:46 AM UTC-8, Nick M. wrote:
"Mega [Andreas Stuermer]" <masterstorm123@gmail.com>: Dec 30 12:17AM -0800
That actually seems doable, but the yeast would grow slower. All the enzymes have been known for a long time. Unless you add methane production for energy making
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On Tue, Dec 30, 2014 at 11:08 AM, <scocioba@gmail.com> wrote:
> Developing microfluidics for rapid protoplasts collection and lysis from
> plant leaf
>
> http://pin.sagepub.com/content/226/1/15.abstract
This is another really cool design. Lots of great ideas!
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On Tue, Dec 30, 2014 at 11:08 AM, <scocioba@gmail.com> wrote:
> Developing microfluidics for rapid protoplasts collection and lysis from
> plant leaf
>
> http://pin.sagepub.com/content/226/1/15.abstract
This is another really cool design. Lots of great ideas!
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they are obviously as dodgy as a 3 pound note, but they usually have what you want and can also be configured to transparently proxy most of the journals web sites.
Great idea. Sure beats spamming the list for my needs.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
From: Tom Knight
Sent: 12/30/2014 2:14 PM
To: diybio@googlegroups.com
Cc: Thomas Knight
Subject: Re: [DIYbio] Yet another paper requestHere you are.
If you want a more predictable set of replies, then I'd recommend the Bioforum group at protocol-online.org. The "Papers" subgroup will find virtually anything, and has people with access to things I can't get. The group is friendly in general.
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Here you are.
If you want a more predictable set of replies, then I'd recommend the Bioforum group at protocol-online.org. The "Papers" subgroup will find virtually anything, and has people with access to things I can't get. The group is friendly in general.
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That actually seems doable, but the yeast would grow slower. All the enzymes have been known for a long time. Unless you add methane production for energy making
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All,Hope everyone had lovely and peaceful holidays.I've been lurking the group for ~16 months now. So far I've done a GFP plant, selective breeding on plants, selective breeding on brewer's yeast, and a few other random projects. I want to thank you all for the invaluable depth of knowledge that is available here.I am a brewer by profession and have recently taken over a brewery with considerable corporate backing, corporate has told me 'Do what you want, don't break anything, and most importantly make great beer.'I take this to me I can build my own synbio lab, I am confident that is within my AOR.I was hoping to receive some input from you all on interesting directions to go with S. cerevisiae.I have seen the iGEM projects on beer, caffeine, limonene(particularly interesting to me), and kumamolisin, and all of those are things I would like to work towards.Any ideas on what I can do in the interim whilst trial running my lab and knowledge base? Looking for projects that would be valuable to me in either an academic or practical regard that would build a good framework of experience to operate on in a professional standard. I also feel indebted to the community and must offer my position to the benefit to the diybio community once it becomes a position of value.Cheers,Nick
On 28 December 2014 at 19:37, Rome Robinson <rxxpr...@gmail.com> wrote:
Are there any DIYBio/Biohacking Youtube Channels? The reason I want to know is because I would like to see what other people in the DIYBio community are doing and also gain information and knowledge.The London Biohackers have a channel but its a bit neglected atm.There are a few things from a while ago;
