Re: [DIYbio] Digest for diybio@googlegroups.com - 10 updates in 3 topics

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• Microfluidics Chat Thread WAS: [DIYbio] Paper request - 1 Update
• Yet another paper request - 7 Updates
• saccharomyces cerevisiae potentialities - 2 Updates

Microfluidics Chat Thread WAS: [DIYbio] Paper request

<scocioba@gmail.com>: Dec 30 11:29PM -0500 See attached photo for an example of my crappy attempt at making pdms more hydrophilic using a UV sterilizer (the breadbox type). 30 minute exposure on both a piranha-treated microscope slide (15min in a solution of 3:1 h2so4:h2o2 *dangerous, corrosive, always use appropriate PPE*) and a freshly minted pdms chip. I dried the slide with a kimwipe and used scotch tape to remove residual dust from the feature side of the pdms chip. Then I just laid the slide and pdms chip (feature side up) into the chamber and set a timer for 30 minutes. Then quickly place the pdms chip feature side down onto the slide and apply gentle pressure around the features. Be sure to not squash the channels.   PDMS was the knock-off ML Solar brand from eBay. 10:1 mix by weight. Degassed in a desiccation chamber to 25+inHg twice and incubated at 60c for 1hr.   Pdms chip was casted in a plastic Petri dish on a shrunk shrinky dink printed using graffix brand shrinkable sheet (white not clear!). Something funky about the clear version. Sticks to everything when in the oven, got better results with white sheets. Printed with a standard brother monochrome laser printer (toner). Chip design taken from template PowerPoint slide from this site:   http://chemistry.beloit.edu/Edetc/nanolab/shrink/index.html   Its a simple gradient generator. Still not as fluid and wettable as I'd like it to be. I used a straw to mouth-vacuum from the outlet port. Aside from some funky phenomena on the rightmost channel, it did seem to separate decently. I used food coloring as the indicator.   Sebastian S. Cocioba CEO & Founder New York Botanics, LLC Plant Biotech R&D   -----Original Message----- From: "Nathan McCorkle" <nmz787@gmail.com> Sent: ‎12/‎29/‎2014 3:21 AM To: "diybio" <diybio@googlegroups.com> Subject: Re: Microfluidics Chat Thread WAS: [DIYbio] Paper request   >> https://raw.githubusercontent.com/nmz787/microfluidic-cad/master/implicitCAD/output/tobacco_mesophyll_protoplast_fusion_device.jpg   > and yeah I know I need to improve the funnel-of-posts angle so the > edges follow the pitch of the posts, so i.e. cells don't get stuck.   Which is demonstrated on the macroscale with marbles: https://www.youtube.com/watch?v=03tx4v0i7MA       -- -Nathan   -- -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en Learn more at www.diybio.org --- You received this message because you are subscribed to the Google Groups "DIYbio" group. To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com. To post to this group, send email to diybio@googlegroups.com. Visit this group at http://groups.google.com/group/diybio. To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9KjSjhbOHkVr-cv3S-TnnNfP8mzvM19q%2BuARWXKEdKhtw%40mail.gmail.com. For more options, visit https://groups.google.com/d/optout.

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Yet another paper request

<scocioba@gmail.com>: Dec 30 02:08PM -0500 I'm lounging around at the museum of natural history and during some downtime I stumbled upon yet another paywalled article about plant protoplasts and microfluidics:   Developing microfluidics for rapid protoplasts collection and lysis from plant leaf   http://pin.sagepub.com/content/226/1/15.abstract   This one is interesting since it works with isolation, single cell sieving, and lysis for DNA extraction all in one chip. Would anyone care to help a broke plant biologist this holiday season? Thanks in advance!   Sebastian S. Cocioba CEO & Founder New York Botanics, LLC Plant Biotech R&D

Tom Knight <tk@mit.edu>: Dec 30 02:14PM -0500

<scocioba@gmail.com>: Dec 30 02:15PM -0500 Great idea. Sure beats spamming the list for my needs.   Sebastian S. Cocioba CEO & Founder New York Botanics, LLC Plant Biotech R&D   -----Original Message----- From: "Tom Knight" <tk@mit.edu> Sent: ‎12/‎30/‎2014 2:14 PM To: "diybio@googlegroups.com" <diybio@googlegroups.com> Cc: "Thomas Knight" <tk@mit.edu> Subject: Re: [DIYbio] Yet another paper request   Here you are. If you want a more predictable set of replies, then I'd recommend the Bioforum group at protocol-online.org. The "Papers" subgroup will find virtually anything, and has people with access to things I can't get. The group is friendly in general.   -- -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en Learn more at www.diybio.org --- You received this message because you are subscribed to the Google Groups "DIYbio" group. To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com. To post to this group, send email to diybio@googlegroups.com. Visit this group at http://groups.google.com/group/diybio. To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/5E30027A-8E1A-4C99-B20A-13103B6080BA%40mit.edu. For more options, visit https://groups.google.com/d/optout.

Tom Hodder <tom@limepepper.co.uk>: Dec 30 07:34PM try these 2 http://sci-hub.org/ http://gen.lib.rus.ec/   they are obviously as dodgy as a 3 pound note, but they usually have what you want and can also be configured to transparently proxy most of the journals web sites.

Tom Hodder <tom@limepepper.co.uk>: Dec 30 07:35PM oops. that was meant to be off list. Sorry.

Nathan McCorkle <nmz787@gmail.com>: Dec 30 05:56PM -0800 > Developing microfluidics for rapid protoplasts collection and lysis from > plant leaf   > http://pin.sagepub.com/content/226/1/15.abstract   This is another really cool design. Lots of great ideas!

Sebastian Cocioba <scocioba@gmail.com>: Dec 30 09:25PM -0500 Yeah the clever cell sorting array is what I'm super curious about. I want to see if my toner printer can handle such small structures using shrinky dinks. Just going to overlay my hemocytometer on the chip and make various posts in a PDMS chip and see what works. Know of any high DPI laser printers for cheap?

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saccharomyces cerevisiae potentialities

mostromundo <mzuorski@gmail.com>: Dec 29 09:50PM -0800 I work in wine production, we want yeast that will consume all of the hexose sugars (especially fructose) and will do so while producing more glycerol and less ethanol. If you can make a fructophilic wine yeast with below-average ethanol production and acceptable sensory characters, you will make a lot of winemakers very happy :)   On Friday, December 26, 2014 9:29:46 AM UTC-8, Nick M. wrote:

"Mega [Andreas Stuermer]" <masterstorm123@gmail.com>: Dec 30 12:17AM -0800 That actually seems doable, but the yeast would grow slower. All the enzymes have been known for a long time. Unless you add methane production for energy making

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