Re: [DIYbio] Good Biotechnology Laboratory Text

I was working on total synthesis of Chlorins and Bacteriochlorins. I didn't work with polymers, mainly just throwing reactants in a pot, waiting, working up reactions and isolating product (chromatography, extraction, etc). Did some NMR to test product purity and identity, which I thought was interesting. Run of the mill organic chemistry really so my biology experience is nonexistent but I have a good understanding of enzyme kinetics, reactions, measurements, and such.

On Monday, December 22, 2014 1:32:27 PM UTC-5, Nathan McCorkle wrote:
A good read is Molecular Cloning by sambrook, there are some here
http://diyhpl.us/~bryan/papers2/bio/books/

Do you have experience with polymer synthesis?

On Sun, Dec 21, 2014 at 7:49 PM, Bryson <bnmc...@ncsu.edu> wrote:
> Hey everyone,
>
> I was wondering if anyone could recommend some good introductory texts to
> applied biotechnology. I only have a BS in Chemistry, but I've had some
> biochemistry as well as having taken 8 credits of introductory biology. On
> the lab techniques side (particularly procedures unique to biology
> experiments) I feel like I would not be able to satisfactorily carry out the
> kind of experiments I'd be most interested in. I did some undergraduate
> research in organic synthesis, but that's obviously not very helpful for
> synthetic biology undertakings. I'm interested in trying to create
> transgenic yeast to produce butanol or other organic products without the
> use of petroleum and other things of that nature.
>
> I was looking at this text, and it seems the closest of anything else I've
> found:
> http://www.amazon.com/gp/product/1405181737/ref=ox_sc_act_title_2?ie=UTF8&psc=1&smid=A33AYO0FZG6YH
>
> I ultimately want to gain some degree of proficiency to where I could
> comfortably begin setting up my own lab to do some experiments in my spare
> time and not waste a lot of money on unnecessary setups, etc. I'd also like
> to get up to speed on the ethics side of things (I don't want to
> inadvertently do something illegal or harmful). How much education (time
> wise) do you think I have ahead of me to be able to undertake this?
>
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--
-Nathan

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