Re: [DIYbio] Re: Uptake of food transgenes by guy bacteria

I think Andreas is right that even describing it at the "organism" level is incorrect, and pandering. We should talk about Genetically Engineered Traits, because we're not making a whole new, unknown and poorly-understood "organism", we're adding a trait to a well known organism.

On 1 December 2014 20:25:02 GMT+00:00, Alec Nielsen <alecnielsen@gmail.com> wrote:
Reclaim the connotation: Genetically enhanced organisms :)



On Mon, Dec 1, 2014 at 3:16 PM, Mega [Andreas Stuermer] <masterstorm123@gmail.com> wrote:
Maybe we can use the power of language and talk of GEOs instead of GMOs. Can you even talk of engineered organisms if you just change one single gene (out of 20000)? It's like saying I "created" a table by hammering a nail into an old table. 

Hm, just foound out that we don't even have a positive word in German. Engineered - there is no verb. 


engineered {adj} {past-p}
technisiert  ---- never used, doesn't fit
genetically engineered {adj} <GE>
gentechnisch verändert   --- verändert means "modified, changed" 
well-engineered {adj}
ausgereift     ---  ausgereift means "well done", but cannot be used in the active form
engineered wood {sg} [wood-based materials]
Holzwerkstoffe {pl}material        literally "wood woking material"
engineered (timber) board
Vollholzplatte {f}constr.
engineered (timber) boards
Platten {pl} auf Vollholzbasis
engineered safety feature <ESF>
sicherheitstechnische Einrichtung {f} [z. B. Kerntechnik]tech.
engineered wooden floor
Fertigparkettboden {m}constr.
engineered wooden flooring
Fertigparkett {n}
genetically engineered food
genmanipulierte Nahrung {f}FoodInd.
genetically engineered foods
gentechnologisch erzeugte Nahrungsmittel {pl}FoodInd.
pre-engineered system [compact system]
Kompaktsystem {n}




On Wednesday, November 12, 2014 11:01:40 PM UTC+1, Sebastian wrote:
I'm giving a talk on food GMOs at the school of visual arts in NYC this weekend and I am preparing some slides and asking people for opinions and whatnot. One conversation I've had had to do with the uptake of transgenes (Bt toxin in particular) by gut bacteria and subsequent expression of said transgene. Now, since I make circuits for plant expression on a daily basis (traditional cloning...sadly), I know that the go-to plant promoter CaMV 35s (cauliflower mosaic virus protein coat promoter region) will barely, if not at all, express in prokaria. The gene does come from bacteria and I am not sure it was codon optimized. That being said, uptake of DNA from the environment by wild type bacteria is commonplace during stress but due to the nature of the internal environment (stomatch/gut), lack of expression, and lack of viability of the Bt toxin in an acidic medium, I am almost certain eating raw GM foods will not produce tiny pesticide factories in the intestines of a human. Whats your thoughts? What are the chances of native gut flora uptaking the dna and expressing the particular protein?

P.S. the goal of the talk is to explain the process of how one produces a transgenic plant, some of the most common traits being introduced, the % acreage of gmo vs traditional farming, the intended function of said transgenes, and how one can process samples of their own foods and test for one element of transgenic crops at home using second hand or open PCR machines, hackerspaces, etc.. Its more for awareness than promoting a side so I don't want to really touch on health issues, environmental damage, etc Just an educational talk explaining the techniques and tests available.

Thanks in advance!

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